Infection and Development of Nosema sp. NIS H5 (Microsporida: Protozoa) in Several Lepidopteran Insects

Japan Agricultural Research Quarterly
ISSN 00213551
NII recode ID (NCID) AA0068709X
Full text

Infection and development of Nosema sp. NIS H5, a microsporidian parasite isolated from Antheraea pernyi, in the cell line of Antheraea eucalypti were investigated, compared with those of Nosema bombycis isolated from the silkworm, Bombyx mori. No differences in the infection rate of A. eucalypti cells were observed by 48 h postinoculation between the two species of Nosema. However, the spread of infection in the cell culture with Nosema sp. became slower than that with Nosema bombycis from 96 h postinoculation onward, resulting in 16% of cell infection with Nosema sp. and 22% of cell infection with N. bombycis at 120 h postinoculation. Morphological changes in the sequence of development were almost the same in both Nosema species, but from 96 h postinoculation onward, the sporogenesis of Nosema sp. was slower than that of N. bombycis. Infectivity test to 13 lepidopteran species revealed that N. bombycis was infectious to Bombyx mori, Bombyx mandarina, Antheraea pernyi, Antheraea yamamai, Hemerophila atrilineata, Spilarctia imparilis, Hyphantria cunea, Glyphodes pyroalis, Leucania separata, Spodoptera litura and Pieris rapae crucivora, but not infectious to Lymantria dispar and Euproctis similis. On the contrary, Nosema sp. was infectious to these 12 lepidopteran species except for B. mori, and highly infectious to L. separata, inducing a lethal infection. Thus, it is concluded that Nosema sp. is not infectious to B. mori and has a wide host range of lepidopteran species, suggesting that Nosema sp. could become a very promising agent for the microbial control of insect pests, especially in sericultural areas.

Date of issued
Creator Syoji HAYASAKA Naoyuki YONEMURA
Available Online
NII resource type vocabulary Journal Article
Volume 33
Issue 1
spage 65
epage 68
Language eng

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