Inducible Expression of Pathogenesis-Related 1 Protein Gene in Transformed Plants
Japan Agricultural Research Quarterly
ISSN | 00213551 |
---|---|
NII recode ID (NCID) | AA0068709X |
Full text
24-2-091-099.pdf1.16 MB
Induced expression of tobacco pathogenesis-related (PR) 1 protein genes was analyzed. The base sequence comparison of the active three genes (PR1a, PR1b and PR1c genes) indicated that there was a well conserved region in 5’ flanking 180 nucleotides in their promotor sequences. Direct gene transfer into tobacco protoplasts by electroporation with the chimeric gene of PR1a promoter and a reporter gene (B-glucuronidase: GUS), and stable transformation of tobacco with a binary vector mediated Agrobacterium infection with the same chimeric gene suggested that the cis-acting element responding to stress or salicylic acid be present in the 0.3Kb sequence of the 5’ flanking region of PR1a gene.
Date of issued | |
---|---|
Creator | Yuko OHASHI Masahiro OHSHIMA and Makoto MATSUOKA |
Available Online | |
NII resource type vocabulary | Journal Article |
Volume | 24 |
Issue | 2 |
spage | 91 |
epage | 99 |
Language | eng |