Evaluation of an Enzyme-Linked Immunosorbent Assay(ELISA) Using Mycobacterium phlei - Absorbed Serum for the Diagnosis of Bovine Paratuberculosis in a Field Study

Japan Agricultural Research Quarterly
ISSN 00213551
NII recode ID (NCID) AA0068709X
Full text

 The specificity of an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of paratuberculous cattle was improved by absorbing the bovine sera with a suspension of Mycobacterium phlei-killed organisms. The newly developed ELISA technique was evaluated and compared with the complement fixation (CF) test for the detection of antibodies to Mycobacterium paratuberculosis. Thirty-eight (66%) of 58 fecal culture positive cattle in a M. paratuberculosis infected herd were positive in the ELISA test, whereas the CF test yielded only 15 (26%) positive results. Calves experimentally infected with M. paratuberculosis seroconverted in the ELISA test at the earlier stage of infection than in the CF test. The ELISA was proposed as an alternative to the CF test for the detection of antibodies against M. paratuberculosis in bovine serum. Application of the ELISA as a diagnostic test for subclinical infection of cattle with M. paratuberculosis was proved to be very useful.

Date of issued
Creator YUICHI YOKOMIZO
Available Online
NII resource type vocabulary Journal Article
Volume 20
Issue 1
spage 60
epage 67
Language eng

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