国立研究開発法人 国際農林水産業研究センター | JIRCAS

The In Vitro Approach to the Cytotoxicity of a Trichothecene Mycotoxin Nivalenol

JARQ : Japan Agricultural Research Quarterly
ISSN
00213551
書誌レコードID(総合目録DB)
AA0068709X
本文フルテキスト

Trichothecene mycotoxins are toxic to leukocytes, and one of the leading symptoms of trichothecene toxicosis is leukopenia. In this study, therefore, to elucidate the underlying mechanism of toxicity, we treated promyelocyte (one of the leukocytes) -derived cell line HL60 with a trichothecene mycotoxin nivalenol for 24 h and investigated the toxin's effects. After treatment with 3 or 10 μg/mL nivalenol, morphologic damage was pronounced. The effect of nivalenol on cell proliferation (5-bromo-2-deoxyuridine (BrdU) incorporation) was examined, and the mean 50% inhibitory concentration was 0.16 μg/mL. At 3 and 10 μg/mL, internucleosomal DNA fragmentation, one of the hallmarks of apoptosis, was apparent. Concentrations of nivalenol-caused morphologic damage are in accordance with DNA fragmentation, indicating that nivalenol-caused morphologic change is due to apoptosis. The media of nivalenol-treated cells contained substantial amounts of interleukin (IL)-8, suggesting that IL-8 contributes to the nivalenol-induced phenomena. Conversely, nivalenol decreased monocyte chemotactic protein-1 secretion. We performed BrdU incorporation to assess the effect of 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid tetraacetoxymethyl ester (BAPTA-AM), which chelates intracellular calcium ion. BrdU incorporation after concomitant treatment with nivalenol and BAPTA-AM was higher than that after treatment with nivalenol alone. Likewise BAPTA-AM considerably attenuated nivalenol-induced IL-8 secretion. Taking both results together, it appears that nivalenol-caused cytotoxicity depends on intracellular calcium ion.

刊行年月2009-01-01
作成者NAGASHIMA HitoshiNAKAGAWA HiroyukiKUSHIRO MasayoIWASHITA Keiko
著者キーワード

cell proliferation

HL60 cell

interleukin-8

intracellular calcium ion

公開者Japan International Research Center for Agricultural Sciences
データ作成日2009-01-01
国立情報学研究所メタデータ主題語彙集(資源タイプ)Journal Article
43
1
開始ページ7
終了ページ11
DOI10.6090/jarq.43.7
権利Japan International Research Center for Agricultural Sciences
言語eng