Allergenicity Forecast Using Immortalized Cells

JIRCAS international symposium series
ISSN 13406108
書誌レコードID(総合目録DB) AA1100908X
本文フルテキスト
Since it is not easy to prepare a standard sera library for allergenicity testing, we planned to develop a new system to evaluate the allergenicity of transgenic plants using the B-cell immortalizing technique. We have developed a method to establish human antibody secreting cells by transforming B-cells with the Epstein-Barr virus(EBV) followed by cell fusion with murine myeloma cells. In our preliminary experiment, we obtained 1,412 multi-clone immortalized human B-cell library stocks from seven healthy donors. The B-cell library contained several kinds of antibody-producing cells. The library stocks may contain antibodies to proteins we have never observed before. It is considered that our new technology will enable the evaluation of the allergenicity of novel proteins derived from less commonly allergenic food or nonfood sources.
作成者 Hiroshi Shinmoto Kazuhiko Nakahara Masuko Kobori Tojiro Tushida
公開者 Japan International Research Center for Agricultural Sciences
オンライン掲載日
5
開始ページ 13
終了ページ 20
言語 eng

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