Development of Chromosome Observation Methods in Acerola (Malpighia glabra L.)

Japan Agricultural Research Quarterly
ISSN 00213551
NII recode ID (NCID) AA0068709X
Full text
A chromosome preparation method using young leaves of acerola (Malpighia glabra) was developed. The young leaves were cut into approximately 2-mm2 for enzymatic maceration and air-drying (EMA). For EMA, an enzyme mixture containing 2% Cellulase Onozuka RS, 1.5% Macerozyme R200 (Yakult), 0.3% Pectolyase Y-23 (Seishin Pharmaceutical Co., Ltd.), and 1 mM EDTA, pH 4.2, at 37°C for 30 min was optimal for chromosome preparation since good preparations, with all 20 chromosomes relatively extended and well-spread without cytoplasm, were observed. There were 11.2 preparations in large leaves (8 to 10 mm long and 3 to 4 mm wide) and 4.2 preparations in small leaves (6 to 8 mm long and 1 to 2 mm wide). Chromomycin A3 (CMA)-positive (+) bands were noted in the telomeric positions of eight chromosomes. 4′-6-diamidino-2-phenylindole (DAPI)-negative bands (−) corresponded to CMA+ bands. The numbers and positions of CMA+ bands were the same in the two cultivars examined: ‘Florida Sweet’ and ‘Sanmi-kei (Hosoba).’ The methods developed in the present study are promising for further cytogenetic studies in acerola.
Date of issued
Creator Masashi YAMAMOTO
Subject Barbados cherry CMA DAPI EMA West Indian cherry
Publisher Japan International Research Center for Agricultural Sciences
Received Date 2024-08-09
Accepted Date 2024-12-16
Available Online
Volume 59
Issue 3
spage 227
epage 231
DOI 10.6090/jarq.24J12
Relation eng
Language eng