The functional properties of edible lavers are well-known; however, there is limited information on the marine red alga Pyropia tenuipedalis, an indigenous species found in the Yamaguchi Prefecture area. In the present study, samples of P. tenuipedalis were collected from the Koto River Estuary and cultivated using land-based cultivation techniques. A methanol/chloroform (M/C) extract obtained from naturally grown and land-based cultivated P. tenuipedalis showed antiallergic properties, including suppression of ear swelling in the Institute of Cancer Research mouse model, inhibition of inflammation-associated enzymes (i.e., phospholipase A₂, lipoxygenase, cyclooxygenase-2, and hyaluronidase), and anti-degranulation in rat basophilic leukemia-2H3 cells. Chemical composition was determined using thin-layer chromatography. Three glyceroglycolipids, namely digalactosyldiacylglycerol, monogalactosyldiacylglycerol, and sulfoquinovosyldiacylglycerol, were detected in the M/C extracts of natural and cultivated algae and were selected as bioactive candidates. The results demonstrate that glyceroglycolipids in natural and cultivated P. tenuipedalis edible algae have promising potential for the development of antiallergic food.