国立研究開発法人 国際農林水産業研究センター | JIRCAS

Analysis of the Genetic Population Structure of <i>Cacopsylla chinensis</i> (Hemiptera: Psyllidae) with Mitochondrial DNA Markers in Saga and Yamaguchi prefectures, Japan

JARQ : Japan Agricultural Research Quarterly
ISSN
00213551
書誌レコードID(総合目録DB)
AA0068709X
本文フルテキスト

DNA sequence analysis of the mitochondrial cytochrome oxidase I (COI)-leucine tRNA (tRNALeu)-cytochrome oxidase II (COII) regions was performed to elucidate the phylogenetic relationships among 465 summer and winter-form psyllids from Saga and Yamaguchi prefectures in Japan. Multiple alignments of these sequences revealed 2 single-nucleotide polymorphisms (SNPs) at nucleotide positions 78 and 258 in the COI and tRNALeu regions, respectively. Based on the nucleotide changes at these loci, these 465 insects were grouped into 3 genotypes: 78A/258A, 78G/258G, and 78G/258A. All but 2 of the 225 insects in Saga belonged to the genotype 78A/258A, and the 2 exceptions belonged to the genotype 78G/258G. Conversely, all the insects (105) collected from Shimonoseki city in western Yamaguchi prefecture belonged to the genotype 78G/258G, while eighty percent of the 135 samples from eastern Yamaguchi prefecture belonged to the genotype 78G/258G. Among the other 27 insect samples, 24 were 78G/258A and 3 were 78A/258A. Using POPTREE2, the psyllids were divided into 5 populations associated with the geographical distribution. The results suggest that the genetic population structure of these psyllids varied widely between Saga and Yamaguchi prefectures.

刊行年月2014-10-01
作成者KATOH HiroshiINOUE HiromitsuUECHI NamiFUJIKAWA TakashiMIYATA Shin-ichiIWANAMI Toru
著者キーワード

cytochrome oxidase I

molecular phylogenetic analysis

Pear psyllids

population genetics

公開者Japan International Research Center for Agricultural Sciences
データ作成日2014-10-01
国立情報学研究所メタデータ主題語彙集(資源タイプ)Journal Article
48
4
開始ページ413
終了ページ417
DOI10.6090/jarq.48.413
権利Japan International Research Center for Agricultural Sciences
言語eng