We conducted a 12-year field experiment to study the combined effects of rice straw compost and chemical fertilizer application on a double rice cropping system in the Mekong Delta, Vietnam and established 7 treatments using a randomized block design with 3 replications. We calculated the yields of treatments relative to those of conventionally fertilized plots (i.e. with no added rice straw compost), and analyzed their annual trends. In the plots with rice straw compost, we observed a positive trend over 12 continuous wet cropping seasons. In comparison with conventional fertilization, the application of rice straw compost with reduced chemical fertilizer can maintain rice productivity over a longer period. We analyzed the nutrient status of the rice straw at harvest over 4 cropping seasons and observed that the Si concentration of the rice straw in conventionally fertilized plots was significantly lower than that in the plots where rice straw compost was applied. The N, P and Mg concentrations of the rice straw increased with the fertilizer application rate, while the concentrations of K, Ca, Mn, Fe, Zn, and Cu did not differ significantly among treatments. Our results suggest that continuous removal of rice straw may reduce the Si availability in soil, thereby decreasing rice productivity. In addition, we verified that rice straw compost can be an effective source of silicon for paddy rice.