国立研究開発法人 国際農林水産業研究センター | JIRCAS

Isolation and Characterization of a Parapoxvirus from Sheep with Papular Stomatitis

JARQ : Japan Agricultural Research Quarterly
ISSN
00213551
書誌レコードID(総合目録DB)
AA0068709X
本文フルテキスト

An outbreak of papular stomatitis occurred in a sheep herd in Hokkaido, Japan. Histological examination, immunohistochemistry, electron microscopic observation, and polymerase chain reaction (PCR) were carried out. Lesions were characterized by epithelial hyperplasia, acanthosis, ballooning degeneration of the spiny layer and stratum granulosum. A parapoxvirus was isolated from the skin lesion of affected sheep and characterized genetically and antigenically. Restriction endonuclease analysis of the PCR product showed an orf virus (ORFV)-specific pattern and the isolate reacted with monoclonal antibodies against ORFV. The partially deduced amino acid sequence of the viral envelope gene was identical to those of the major Japanese ORFVs. These results indicated that the outbreak was due to infection by parapoxvirus. The isolated virus could be classified into ORFV, and was closely related to the major Japanese ORFVs, but not foreign ORFVs or other parapoxviruses.

刊行年月2005-07-01
作成者KANOU YukikoINOSHIMA YasuoSHIBAHARA TomoyukiISHIKAWA YoshiharuKADOTA KoichiOHASHI SeiichiMORIOKA KazukiYOSHIDA KazuoYAMADA Shunji
著者キーワード

orf virus

outbreak

公開者Japan International Research Center for Agricultural Sciences
データ作成日2005-07-01
国立情報学研究所メタデータ主題語彙集(資源タイプ)Journal Article
39
3
開始ページ197
終了ページ203
DOI10.6090/jarq.39.197
権利Japan International Research Center for Agricultural Sciences
言語eng