国立研究開発法人 国際農林水産業研究センター | JIRCAS

Digoxigenin (DIG)-Based AFLP Analysis of Three Citrus Cultivars

JARQ : Japan Agricultural Research Quarterly
ISSN
00213551
書誌レコードID(総合目録DB)
AA0068709X
本文フルテキスト

A digoxigenin (DIG)-based amplified fragment length polymorphism (AFLP) method was developed to distinguish three closely related citrus cultivars, ‘Kishu’, ‘Mukaku-Kishu’ and ‘Hira-Kishu’. In particular, ‘Mukaku-Kishu’ characterized by seedless fruit is very important for breeding purposes. Initial attempts at using the DIG-based RAPD procedure failed to detect ‘Mukaku-Kishu’-specific bands. However, the DIG-based AFLP method enabled to detect such bands, indicating that this method is more sensitive than the DIG-based RAPD procedure. The detailed protocol for the DIG-based AFLP methods is presented in this paper.

刊行年月2003-10-01
作成者HANADA HiromiKAYANO ToshiakiKOGA-BAN YasunoriTANAKA HiroshiTABEI Yutaka
著者キーワード

PCR

RAPD

fingerprinting

公開者Japan International Research Center for Agricultural Sciences
データ作成日2003-10-01
国立情報学研究所メタデータ主題語彙集(資源タイプ)Journal Article
37
4
開始ページ225
終了ページ228
DOI10.6090/jarq.37.225
権利Japan International Research Center for Agricultural Sciences
言語eng