国立研究開発法人 国際農林水産業研究センター | JIRCAS

Preparation of Microprotoplasts for Partial Genome Transfer via Microprotoplast Fusion in Liliaceous Ornamental Plants

JARQ : Japan Agricultural Research Quarterly
ISSN
00213551
書誌レコードID(総合目録DB)
AA0068709X
本文フルテキスト

We aimed to produce intergeneric hybrid plants with only one or a few alien chromosomes via microprotoplast fusion for genetic improvement and chromosome studies in Liliaceous ornamental plants. In order to apply this technique, it is essential to establish an efficient system for mass-preparation of microprotoplasts. We have established 2 different systems for isolating microprotoplasts, one from partially synchronized cell suspension cultures of Hemerocallis hybrida and the other from developing microspores of Lilium longiflorum. Here, the induction of micronucleated cells, isolation of microprotoplasts, and enrichment of smaller microprotoplasts containing one or a few chromosomes are described for both systems.

刊行年月2002-07-01
作成者SAITO HiroyukiNAKANO Masaru
著者キーワード

cell suspension culture

Hemerocallis hybrida

Lilium longiflorum

micronucleation

microsporocyte

公開者Japan International Research Center for Agricultural Sciences
データ作成日2002-07-01
国立情報学研究所メタデータ主題語彙集(資源タイプ)Journal Article
36
3
開始ページ129
終了ページ135
DOI10.6090/jarq.36.129
権利Japan International Research Center for Agricultural Sciences
言語eng