Separation and Identification of Desulfoglucosinolates in Japanese Rapessed by LC/APCI-MS

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X
本文フルテキスト

To identify seed glucosinolates in Japanese rapeseed (Brassica napus L.), a method for the separation and identification of various desulfoglucosinolates that were prepared by digestion with sulfatase from glucosinolates, was developed using liquid chromatography/atmospheric pressure chemical ionization mass spectrometry (LC/APCI-MS). Desulfoglucosinolates were separated by a high-performance liquid chromatography (HPLC) system and were analyzed by APCL-MS. Nine desulfoglucosinolates were easily identified from the protonated molecular ions (M + N)+ and the group-specific fragment ions (M + H - C6H10O5)+. The seed of Japanese rapeseed contained the same glucosinolates as the exotic cultivars. These results revealed that LC/APCI-MS analysis is suitable for the identification of desulfoglucosinolates. HPLC analysis showed that there were remarkable varietal differences in the total and individual glucosinolate contents among the 50 main Japanese cultivars. Among these cultivars, Norin 18 showed the lowest glucosinolate content.

刊行年月日
作成者 Masahiko ISHIDA Ichimi CHIBA Yoshinao OKUYAMA Yoshihito TAKAHATA Norihiko KAIZUMA
オンライン掲載日
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
31
2
開始ページ 73
終了ページ 80
言語 eng

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