国立研究開発法人 国際農林水産業研究センター | JIRCAS

Genetic Relatedness among the Pathogenic Variants in Fusarium oxysporum Causing Wilts of Cucurbits

JARQ : Japan Agricultural Research Quarterly
ISSN
00213551
書誌レコードID(総合目録DB)
AA0068709X
本文フルテキスト

Strains of 5 cucurbit-infecting formae speciales of Fusarium oxysporum were collected from various locations in Japan. The assay for pathogenicity showed the complexity of their infection spectra. In some cases, a single forma specialis infected multiple genera or species of the family Cucurbitaceae, and different formae speciales shared the same host plants. In other cases, a single forma specialis contained subgroups, designated as races, differing in pathogenicity to a set of differential cultivars within the same plant species. Genetic relatedness among strains of the cucurbit-infecting F. oxysporum was determined by DNA fingerprinting with the fungal nuclear repetitive DNA sequences. Four repetitive DNA sequences used as probes were chosen from a genomic library of F. oxysporum f. sp. lagenariae and designated as FOLR clones. DNA fingerprinting of total DNA of strains from the cucurbit-infecting formae speciales after hybridization with FOLR sequences enables to distinguish strains not only at the forma specialis level but also at the race level. On the basis of DNA fingerprint profiles, phylogenetic relationships among the strains were analyzed by parsimony and UPGMA methods. The analyses indicated that the formae speciales and the races are intraspecific variants specialized in DNA level as well as host range, and that FOLR DNA fingerprinting can be used for the differentiation of these pathogenic veriants.

刊行年月1996-07-01
作成者Fumio NAMIKI Kazufumi NISHI Takashi TSUGE
データ作成日1996-07-01
国立情報学研究所メタデータ主題語彙集(資源タイプ)Journal Article
30
3
開始ページ159
終了ページ165
言語eng