A New Method for Estimation Quality Deterioration of Agricultural Product

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X
本文フルテキスト

A new method has been development to measure the amount of 1, 3-diethyl-2-thiobarbituric acid (DETBA)-malondialdehyde (MDA) adduct as an index of lipid peroxidation. The DETBA-MDA adduct is extracted with ethyl acetate and the amount is measured by spectrofluorometry of highperformance liquid chromatography with a fluorescence detector. The DETBA method which is highly sensitive, and specific, is as simple as the traditional colorimetric TBA method, and enables to measure DETBA values from several agricultural products. The use of the DETBA method enabled to discriminate slight differences in garden peas with external deterioration that was hardly recognizable with the naked eye. The increase in the DETBA values during storage of the garden peas was closely related to the change in surface color and the decrease in the contents of both chlorophyll and reduced form of (or total) ascorbic acid contents. Similar observation was also made in sweetpotatoes. These results suggest that the DETBA method is suitable for estimating quality deterioration during the storage of agricultural products.

刊行年月日
作成者 Ikuo SUDA Shu FURUTA Yoichi NISHIBA
オンライン掲載日
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
29
4
開始ページ 207
終了ページ 213
言語 eng

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