Analysis of the Genetic Population Structure of Cacopsylla chinensis (Hemiptera: Psyllidae) with Mitochondrial DNA Markers in Saga and Yamaguchi prefectures, Japan
ISSN | 00213551 |
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NII recode ID (NCID) | AA0068709X |
DNA sequence analysis of the mitochondrial cytochrome oxidase I (COI)-leucine tRNA (tRNALeu)-cytochrome oxidase II (COII) regions was performed to elucidate the phylogenetic relationships among 465 summer and winter-form psyllids from Saga and Yamaguchi prefectures in Japan. Multiple alignments of these sequences revealed 2 single-nucleotide polymorphisms (SNPs) at nucleotide positions 78 and 258 in the COI and tRNALeu regions, respectively. Based on the nucleotide changes at these loci, these 465 insects were grouped into 3 genotypes: 78A/258A, 78G/258G, and 78G/258A. All but 2 of the 225 insects in Saga belonged to the genotype 78A/258A, and the 2 exceptions belonged to the genotype 78G/258G. Conversely, all the insects (105) collected from Shimonoseki city in western Yamaguchi prefecture belonged to the genotype 78G/258G, while eighty percent of the 135 samples from eastern Yamaguchi prefecture belonged to the genotype 78G/258G. Among the other 27 insect samples, 24 were 78G/258A and 3 were 78A/258A. Using POPTREE2, the psyllids were divided into 5 populations associated with the geographical distribution. The results suggest that the genetic population structure of these psyllids varied widely between Saga and Yamaguchi prefectures.
Date of issued | |
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Creator | KATOH Hiroshi INOUE Hiromitsu UECHI Nami FUJIKAWA Takashi MIYATA Shin-ichi IWANAMI Toru |
Subject |
cytochrome oxidase I molecular phylogenetic analysis Pear psyllids population genetics |
Publisher | Japan International Research Center for Agricultural Sciences |
Available Online | |
NII resource type vocabulary | Journal Article |
Volume | 48 |
Issue | 4 |
spage | 413 |
epage | 417 |
DOI | 10.6090/jarq.48.413 |
Rights | Japan International Research Center for Agricultural Sciences |
Language | eng |