Analysis of the Genetic Population Structure of Cacopsylla chinensis (Hemiptera: Psyllidae) with Mitochondrial DNA Markers in Saga and Yamaguchi prefectures, Japan

Japan Agricultural Research Quarterly
ISSN 00213551
NII recode ID (NCID) AA0068709X

DNA sequence analysis of the mitochondrial cytochrome oxidase I (COI)-leucine tRNA (tRNALeu)-cytochrome oxidase II (COII) regions was performed to elucidate the phylogenetic relationships among 465 summer and winter-form psyllids from Saga and Yamaguchi prefectures in Japan. Multiple alignments of these sequences revealed 2 single-nucleotide polymorphisms (SNPs) at nucleotide positions 78 and 258 in the COI and tRNALeu regions, respectively. Based on the nucleotide changes at these loci, these 465 insects were grouped into 3 genotypes: 78A/258A, 78G/258G, and 78G/258A. All but 2 of the 225 insects in Saga belonged to the genotype 78A/258A, and the 2 exceptions belonged to the genotype 78G/258G. Conversely, all the insects (105) collected from Shimonoseki city in western Yamaguchi prefecture belonged to the genotype 78G/258G, while eighty percent of the 135 samples from eastern Yamaguchi prefecture belonged to the genotype 78G/258G. Among the other 27 insect samples, 24 were 78G/258A and 3 were 78A/258A. Using POPTREE2, the psyllids were divided into 5 populations associated with the geographical distribution. The results suggest that the genetic population structure of these psyllids varied widely between Saga and Yamaguchi prefectures.

Date of issued
Creator KATOH Hiroshi INOUE Hiromitsu UECHI Nami FUJIKAWA Takashi MIYATA Shin-ichi IWANAMI Toru
Subject

cytochrome oxidase I

molecular phylogenetic analysis

Pear psyllids

population genetics

Publisher Japan International Research Center for Agricultural Sciences
Available Online
NII resource type vocabulary Journal Article
Volume 48
Issue 4
spage 413
epage 417
DOI 10.6090/jarq.48.413
Rights Japan International Research Center for Agricultural Sciences
Language eng

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