Efficient Transgene Expression in Chrysanthemum, Chrysanthemum morifolium Ramat., with the Promoter of a Gene for Tobacco Elongation Factor 1 α Protein
ISSN | 00213551 |
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NII recode ID (NCID) | AA0068709X |
We investigated the usefulness of the promoter of a gene for tobacco elongation factor 1α protein (EF1 α) for transgene expression in the chrysanthemum (Chrysanthemum morifolium Ramat.). The EF1 α promoter was fused to the β-glucuronidase gene (gus) and introduced into the chrysanthemum. We obtained 238 putative transformants and GUS assay of the leaves of the in vitro plants revealed that 29.0% (69/238) of the putative plants were GUS-positive. The plants in the greenhouse that were 20 months after regeneration still showed a GUS activity in their leaves and petals. The tobacco EF1 α promoter expressed the transgene more efficiently than the 35S promoter of Cauliflower mosaic virus and could be used for transgene expression in chrysanthemum.
Date of issued | |
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Creator | AIDA Ryutaro NAGAYA Shingo YOSHIDA Kazuya KISHIMOTO Sanae SHIBATA Michio OHMIYA Akemi |
Subject |
Agrobacterium tissue culture transformation ornamental plants |
Publisher | Japan International Research Center for Agricultural Sciences |
Available Online | |
NII resource type vocabulary | Journal Article |
Volume | 39 |
Issue | 4 |
spage | 269 |
epage | 274 |
DOI | 10.6090/jarq.39.269 |
Rights | Japan International Research Center for Agricultural Sciences |
Language | eng |